Initially designed by Oscorp Industries to collect all cross-species in a more efficient way than the S-01, the S-02 was reprogrammed with one purpose: slay Spider-Man. Spider-Man encountered the S-02 after escaping from Oscorp. Curt Connors' first-drafted cure had paralyzed Alistaire Smythe, causing him to send the S-02 after Spider-Man (after knowing that he and Spidey were working together). After the web-slinger and the giant robot dueled (which caused tons of damage and nearly killing TV reporter Whitney Chang, who was rescued by Spider-Man once again), the two battled atop Oscorp Tower (with Spider-Man's déjà vu rising) with the hero finally defeating the robotic monster by jamming a radio antenna through its mouth, destroying it.
The S-02 can burrow into the ground and propel itself at high speed to reach its prey, with a bit of collateral damage along the way.